The Luminex technology is a bead-based immunoassay that allows for multiplex detection of up to 100 analytes simultaneously. Color-coded microspheres, or beads, are internally dyed with different proportions of red and infrared fluorophores that correspond to a distinct spectral signature, or bead region. The quantification of multiple cytokines and other biomarkers in a sample provides critical information about biological processes and diseases. The Luminex system produces results comparable to conventional assays such as ELISA and qPCR but with greater efficiency and throughput.
Luminex multiplex assays utilize color-coded superparamagnetic beads coated with analyte-specific antibodies. Beads recognizing different target analytes are mixed together and incubated with the sample. Captured analytes are subsequently detected using a cocktail of biotinylated detection antibodies and a streptavidin-phycoerythrin conjugate.
Luminex used for protein assay and RNA assay for 80 protein targets and 80 RNA gene expression targets from complex biological samples respectively.
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