The Hands-On RT-PCR Workshop is an intensive training program designed to provide participants with practical experience and a strong foundation in the techniques of RT-PCR. RT-PCR is a fundamental molecular biology tool used to amplify and quantify RNA molecules, making it invaluable in various research and clinical applications. This workshop aims to equip participants with the skills necessary to execute and analyze RT-PCR experiments accurately and effectively.

The workshop will be led by experienced molecular biologists who have a strong background in RT-PCR techniques and their applications. They will guide participants through hands-on activities, provide insights into best practices, and address any questions or challenges.

What is RT-PCR?
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR in real time. Real-time PCR can be used quantitatively and semi-quantitatively.

Workshop Objectives:

  1. Overall lab orientation as offline (includes – a lecture on basic real-time PCR, basic lab hygiene, good laboratory practice, an overview on a QuantStudio 5 - RT-PCR), lab visit - introduction to lab and lab instruments, SOP reading.
  2. Observation of Real-Time PCR work, if any. Demo training/practice of pipetting and QS5 software operation.
  3. Assay set up for cell treatment, cell harvesting, and storage, RNA extraction by Trizol method, cDNA synthesis/preparation..
  4. Assay preparation, Real-Time PCR by SYBR Green chemistry, data acquisition and analysis, certification after successful completion of training.

Workshop Details:

Duration: 2 Weeks of training, combining hands-on sessions, and interactive discussions.
Location: Conveniently located in Aundh, Pune.
Materials Provided: Please refer the brochure for details

More Details Click Here

Workshop Format: The workshop will be conducted over 2 weeks, combining hands-on laboratory sessions, and interactive discussions. Participants will work with cutting-edge RT-PCR instruments and software platforms, gaining practical experience that reinforces theoretical concepts.

Who Should Attend: This workshop is suitable for researchers, scientists, and students who have a basic understanding of molecular biology and want to enhance their skills in RT-PCR techniques.

The Hands-On RT-PCR Workshop offers participants a unique opportunity to acquire practical skills and in-depth knowledge of RT-PCR techniques. On completion of the workshop, participants will be well-equipped to execute and analyze RT-PCR experiments. Register now to secure your spot.

Frequently Asked Questions

Q. What is the timing for the training?
Ans:   10.30 am to 6 pm

Q. What is the schedule for the IABRD training modules?
Ans:   Three modules we posted on our website -
          1. Animal cell culture
          2.Flow cytometry
          3. RT-PCR
          These three modules are scheduled in the first month, second, and third month of each quarter respectively.

Q. What is the eligibility criteria for Basic RT-PCR?
Ans:   At least a final year undergraduate student

Q. How long does the Basic RT-PCR Hands-on Training course take?
Ans:   2 Weeks

Q. How many days do we have to come in a week for training?
Ans:   Five days a week - (Monday to Friday)

Q. Will we get hands-on experience during training?
Ans:   Yes, As per the available time at the time of training

Q. Will the certificate be provided?
Ans:   Yes, After successfully completing the training and the short viva.

Q. How many experiments are covered in the training period?
Ans:   As per the provided training module or brochure.

Q. Will I receive the guidance to write the research paper?
Ans:   No

Q. Can I use the generated raw data for personal use?
Ans:   No, the generated Raw data is only for training purposes only

Q. Can I publish my work?
Ans:   No, you cannot publish your work until you receive permission from HOD, directors.

Q. Can I take Photos of the results?
Ans:   No, Photography is strictly prohibited.

Q. What is the fee structure of the Basic RT-PCR?
Ans:   Please Refer to the respective brochure or connect with us via Email at or contact us at 8380036211, for more information.

Q. Is the fee inclusive of GST?
Ans:   No, course fees do not include GST.

Q. If I still have some questions, who can I speak to?
Ans:   Please contact us using the WhatsApp feature available at our website
          Email on
          Call us at 8380036211 for further information.

Q. I am unable to email you, what should I do?
Ans:   Please contact us using the WhatsApp feature available at our website
          Call us at 8380036211 for further information.